208: Ultraviolet C Inactivation of Salmonella Enteritidis in Raw Chicken Juice on Food Contact Surfaces Using the Contamination Sanitization Inspection and Disinfection (CSI-D+) Device
Information
Introduction
Salmonella is the leading cause of bacterial foodborne illness in the United States. The Contamination Sanitization Inspection and Disinfection (CSI-D+) device is a handheld portable system equipped with fluorescence imaging with dual operations of detection and disinfection of microorganisms on food contact surfaces. The aim of this research was to assess the effectiveness of the CSI-D+ device for the inactivation of bacteria in food residue on common food contact surfaces found in food processing environments.
Methods
Raw chicken juice was inoculated with Salmonella enterica serovar Enteritidis at a concentration of 108 CFU/ml and introduced to stainless steel coupons. Next, the stainless steel coupons were exposed at low intensity (5 mW/cm²) UV-C wavelength (275 nm) using the CSI-D+ device for 15 s. The levels of S. Enteritidis, Escherichia coli, coliforms, and aerobic plate count (APC) following treatment were compared to untreated controls.
Results
Reductions of 58.5%, 60%, and 64% were observed for S. Enteritidis, E. coli, coliforms, and APC following treatment of inoculated stainless steel at low intensity for 15 s. Additional trials will be conducted to assess the effectiveness of the CSI-D+ device under different settings and on various surfaces. The results of this trial show that UV-C exposure of at least 15 s reduces the growth of S. Enteritidis by > 50% on stainless steel.
Significance
The application of UV-C radiation on pathogens within food matrixes on FCS to deter biofilm formation has not been fully explored. Moreover, this is the first study to assess the disinfection module of the CSI-D+ on FCS. The successful application of the CSI-D+ as a disinfection tool would help commercialize this device in private food processing facilities to maintain and regulate food safety.
Authors: G. Tirpude, G. Cho, J. Van Kessel, J. Sonnier, B. Haley, J. Qin, M. Kim, F. Vasefi, S. Sokolov, R. S. Hellberg
