262: High-Calorie Obesity Simulation With Chinese Sausage: Evaluating Resveratrol Butyrate Derivatives for Weight Loss and Metabolic Syndrome Management

Introduction

This study evaluated the potential of 3-O-butanoylresveratrol (ED4), a monoester derivative of resveratrol butyrate esters, in managing obesity and metabolic syndrome using a high-calorie diet model based on Chinese sausage.

Methods

The model effectively induced obesity-related phenotypes, including increased body weight (BW), body fat, dysregulated blood lipids, and altered short-chain fatty acids (SCFAs) levels in stool and serum. ED4 supplementation (20 mg/kg BW/day) for five weeks significantly mitigated BW gain, reduced body fat accumulation, and improved blood lipid profiles. SCFA dysregulation was also corrected.

Results

Mechanistically, ED4 enhanced metabolic regulation by increasing PPAR-γ and reducing SREBP-1C mRNA expression, thereby inhibiting fat deposition and lipid overproduction. Additionally, a novel interaction between gut microbiota and adipose tissue metabolism was identified, suggesting that ED4's effects are mediated in part through gut microbial modulation. Contrary to expectations, ED4 supplementation alleviated metabolic abnormalities induced by the high-calorie diet while promoting gut-driven metabolic benefits.

Significance

These findings demonstrate the efficacy of ED4 as a promising candidate for weight management and metabolic syndrome mitigation, offering insights into food-derived strategies for addressing diet-induced obesity.

Authors: Ming-Kuei Shih, Cheng-Kai Shie, Chih-Yao Hou, Yu-Wei Chen, Shin-Yu Chen, Li-Jung Yin, Bao-Hong Lee, Ping-Hsiu Huang