337: Effect of UV-C and Holder Pasteurization on Anti-Adhesive Activity of Bovine Lactoferrin

Introduction

E. coli is the one of the pathogenic intestinal microorganisms that make our gut microbiome unhealthy. And there are multiple ways to keep gut microbiome healthy like prebiotics and probiotics, antibiotics supplementation, etc. However, the emergence of antimicrobial-resistant strains poses a significant challenge. This study will check the antiadhesive activity of different concentrations of lactoferrin for UV-C processing and Holder Pasteurization.

Methods

Caco-2 cells were seeded in 24 well plates at the rate of 105cells/cm2 in freshly prepared media and then plates were kept in incubator at 37°C and 5% CO2 until cells got differentiated completely and form a confluent monolayer. The confluent monolayers of Caco-2 cells were treated with 1mL of different concentrations of lactoferrin solution in MEM with 10% FBS. After letting cells and lactoferrin bind to each other, 1 mL of bacterial suspension in logarithmic phase was added to the each well, and let bacteria adhere to the cells for 1 in incubator at 37°C and 5% CO2. After this, unbounded E. coli and lactoferrin removed from the monolayers by washing with Phosphate Buffered Saline five times. The adhered bacteria to the cells were cultured on selective media and CFU were counted after lysis of monolayer with 500 µL of 0.1% ice cold Triton X.

Results

In lactoferrin with concentration of 1mg/ml 60% of E. coli inhibit the adherence to the caco-2 cells while for the concentration of 0.5mg/ml only 2% of E coli inhibit the adherence to the caco-2 cells. There was significant difference (p < 0.05) observed for the percentage anti adhesive activity of lactoferrin for E. coli. The results for UV-C and Holder Pasteurization processes lactoferrin is under process.

Significance

Antiadhesive strategies are one of the promising methods for preventing microbial infections by blocking the binding of microbes to gut epithelial cells, and could also reduce the development of resistant microorganisms.

Authors: Sulabh Singh, David Dallas, Rohit Kumar, Bishal Barman